Making point mutations in Escherichia coli BL21 genome using the CRISPR-Cas9 system

Editing the Mouse Genome Using the CRISPR-Cas9 System.

The ability to modify the murine genome is perhaps one of the most important developments in modern biology. However, traditional methods of genomic engineering are costly and relatively clumsy in their approach. The use of programmable nucleases such as zinc finger nucleases and transcription activator-like effector nucleases significantly improved the precision of genome-editing technology, b...

Efficient Genome Editing in Apple Using a CRISPR/Cas9 system

Genome editing is a powerful technique for genome modification in molecular research and crop breeding, and has the great advantage of imparting novel desired traits to genetic resources. However, the genome editing of fruit tree plantlets remains to be established. In this study, we describe induction of a targeted gene mutation in the endogenous apple phytoene desaturase (PDS) gene using the ...

Ethical Issues in Genome Editing using Crispr/Cas9 System

This article reviews ethical issues related to genome editing using CRISPR/Cas9 system. The use of CRISPR/ cas9 revives many previous social and ethical issues with humans, other organisms and the environment, such as taking into account the non-maleficence principle in risk assessment, genome editing in germline, safety issues to avoid ecological impairment or the possible use of the technique...

Genome-wide targeted mutagenesis in rice using CRISPR/Cas9 system

Complete Genome Sequence of Escherichia coli Strain BL21

Escherichia coli strain BL21 is one of the widely used bacterial hosts for high-level recombinant protein production and for other applications. Here, we present the complete genome sequence of a commercial version of the Escherichia coli BL21 strain.